BrightLine®图像拆分二向色分束器
当通过颜色分离光束以同时捕获多个图像时，这些分束器为透射光和反射光提供*的图像质量。对于诸如(FRET)和实时活细胞成像这样的应用，用户现在可以将两种、四种甚更多的颜色分别放到多个相机或单个照相机传感器的多个区域中。这些滤光片的出色平坦度实际上消除了大多数常见成像系统反射光束的像差(参见第63页的技术说明)。
BrightLine®图像拆分二向色分束器
标准边缘波长 | 普通荧光团对分裂 | 平均反射带 | 平均透射带 | 尺寸 (L x W x H) | 滤光片型号 |
484 nm | DAPI/FITC (or BFP/GFP) | 350 – 475 nm | 492.3 – 950 nm | 25.2 x 35.6 x 1.05 mm | FF484-FDi01-25x36 |
509 nm | CFP/YFP | 350 – 500 nm | 518.3 – 950 nm | 25.2 x 35.6 x 1.05 mm | FF509-FDi01-25x36 |
538 nm | GFP/mOrange | 350 – 528.4 nm | 547.7 – 950 nm | 25.2 x 35.6 x 1.05 mm | FF538-FDi01-25x36 |
560 nm | YFP/dTomato | 350 – 550 nm | 570.1 – 950 nm | 25.2 x 35.6 x 1.05 mm | FF560-FDi01-25x36 |
580 nm | GFP/mCherry (or FITC/TxRed) | 350 – 570 nm | 590.8 – 950 nm | 25.2 x 35.6 x 1.05 mm | FF580-FDi01-25x36 |
640 nm | Cy3/Cy5 | 350 – 629.5 nm | 652 – 950 nm | 25.2 x 35.6 x 1.05 mm | FF640-FDi01-25x36 |
662 nm | TxRed/Cy5 | 350 – 650 nm | 673.7 – 950 nm | 25.2 x 35.6 x 1.05 mm | FF662-FDi01-25x36 |
Image Splitting Dichroic Beamsplitters Common Specifications
属性 | Value | Comment |
Transmission | > 93% | Averaged over the specified band |
Reflection | > 95% | Averaged over the specified band |
Flatness | < λ / 4 Peak-to-valley at λ = 633 nm | Spherical error measured over a 10 mm aperture |
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